s pneumoniae atcc 51916 64  (ATCC)

Journal: Nature Communications

Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin

doi: 10.1038/s41467-025-67099-4

Figure Lengend Snippet: A Flow cytometry analysis of 0.5 μM mItln2 binding to mouse fecal samples under Ca 2+ and EDTA conditions plotted as lectin binding (anti-Strep DY549) vs. SSC. B Images of murine fecal samples stained with 0.5 μM mItln2 (magenta) in the presence of EDTA (top) or Ca 2+ (bottom) and counterstained with SYTO BC (teal). mItln2 was detected by Strep antibody. Scale bars, 10 μm. ( C , D ) Flow cytometry analysis of 0.6 μM hItln1 and mItln2 binding to L. reuteri under Ca 2+ and EDTA conditions. Dot plots ( C ) show lectin binding (anti-Strep DY549) vs. nucleic acid stain (SYTO BC). Histogram ( D ) displays cell counts as a percent of the maximum signal against lectin binding in different conditions. ( E , F ) Flow cytometry analysis of mItln2 binding to K. pneumoniae UCI60 ( E ) and L. johnonii RJX009 ( F ) under Ca 2+ or EDTA conditions. MItln2 was detected by Strep antibody. Unstained samples (no lectin) served as controls. Data in ( A ), ( C ), ( D ), and ( E ) are representative of three independent experiments. Data in ( B ) and ( F ) are representative of two independent experiments.

Article Snippet: S. pneumoniae (Klein) Chester serotypes 70 (ATCC10370) were obtained from ATCC.

Techniques: Flow Cytometry, Binding Assay, Staining

Journal: Nature Communications

Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin

doi: 10.1038/s41467-025-67099-4

Figure Lengend Snippet: A SSC-A vs. FSC-A analysis of L. reuteri DSM20016 treated with 5 μM each of hItln1 and mItln2. Untreated bacteria and SYTO BC-labeled bacteria served as controls. B Histogram plot displaying cell counts as a percent of the maximum signal against SSC-A for data in ( A ). C Brightfield microscopy of L. reuteri DSM20016 after 3-h treatment with 5 μM mItln2. Red arrows indicate loss of cell integrity. Untreated bacteria served as a control. Scale bars, 10 μm. D Time-lapse images of L. reuteri DSM20016 following treatment with 5 μM mItln2 (red) in the presence of Ca 2+ and counterstained with SYTO BC (green). Scale bars, 20 μm. E Quantification of viable L. reuteri DSM20016 by dilution plating after incubation with various concentrations of mItln2 for 4 h. Data show mean ± SEM ( n = 3 independent experiments; one-way ANOVA followed by Dunnett’s multiple comparisons test). F Growth curve of K. pneumoniae UCI60 in the presence or absence of mItln2 at varying concentrations. Data show mean ± SEM ( n = 3 independent experiments). Untreated microbes served as controls. ( G , H ) Assessment of disruption of lactose-functionalized liposome, encapsulating Texas red-dextran dye, after treatment with mItln2 for 1 h. Spectroscopic assay ( G ) measured increase in absorbance at 405 nm following mItln2 treatment, with unfunctionalized liposomes serving as a control. Data are shown as mean ± SD ( n = 3 technical replicates). Microscopy images ( H ) revealed dye leakage and loss of liposome integrity. Scale bars, 10 μm. Data in ( C ), ( D ), ( F ), ( G ) and ( H ) are representative of two independent experiments. Data in ( A ) and ( B ) are representative of three independent experiments. Source data are provided as source data file.

Article Snippet: S. pneumoniae (Klein) Chester serotypes 70 (ATCC10370) were obtained from ATCC.

Techniques: Bacteria, Labeling, Microscopy, Control, Incubation, Disruption, Liposomes

Journal: Nature Communications

Article Title: Intelectin-2 is a broad-spectrum antimicrobial lectin

doi: 10.1038/s41467-025-67099-4

Figure Lengend Snippet: A Flow cytometry analysis of 0.5 μM hItln2 binding to human fecal samples under Ca 2+ and EDTA conditions, plotted as lectin binding (Streptactin DY549) vs. SSC). B Microscopy images of human fecal samples stained with 5 μM hItln2 (magenta) in the presence of Ca 2+ or EDTA and counterstained with SYTO BC (teal). hItln2 was detected by Streptactin. Scale bars, 10 µm. C Flow cytometry analysis of 1 μM hItln2 binding to S. aureus MRSA under Ca 2+ and EDTA conditions, plotted as lectin binding (anti-Strep DY549) vs. SSC. Treatment with SYTO BC and Strep antibody (without lectin) served as a control. D Time-lapse images of S. aureus MRSA treated with 5 μM hItln2 in the presence of Ca 2+ and counterstained with SYTO BC (green). Examples of cell agglutination are indicated with white arrowheads. Untreated bacteria served as control. Scale bars, 10 µm. E Viable S. aureus MRSA quantified by dilution plating after incubation with various concentrations of hItln2 for 4 h. Data represent mean ± SEM (n = 3 independent experiment; one-way ANOVA followed by Dunnett’s multiple comparisons test). F Flow cytometry analysis of hItln2 binding to K. pneumoniae UCI60 under Ca 2+ or EDTA conditions, detected by Streptactin. Treatment with SYTO BC (without lectin) served as a control. G Growth curve of K. pneumoniae UCI60 in the presence or absence of hItln2 at varying concentrations. Data show mean ± SEM ( n = 3 independent experiments). Data in ( A ), ( B ), ( C ), ( D ), and ( F ) are representative of three independent experiments. Source data are provided as source data file.

Article Snippet: S. pneumoniae (Klein) Chester serotypes 70 (ATCC10370) were obtained from ATCC.

Techniques: Flow Cytometry, Binding Assay, Microscopy, Staining, Control, Agglutination, Bacteria, Incubation